FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3975277
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1704.0563464E-927.6644631
TACTCGG10350.027.2467273
CTACTCG10200.026.2650432
CGAGTAG11850.023.7977753
GCTGGGA89250.022.6963549
CCGAGTA11250.022.560292
GAGTAGC26300.019.8365084
CCCAAAG68300.019.417781
GTAGCTG41100.019.0973286
AGTAGCT43350.019.0818965
CTCGGGA14300.019.0629435
TCGGGAG15000.018.8000056
GGAGGCT65050.018.7850829
CCCGAGT15500.018.5084191
GTGCTGG62650.018.3798947
AAAGTGC57450.018.3255044
TTGGGAG59250.018.1654056
CCAAAGT64200.018.0827882
AGTGCTG62200.018.059496
TAGCTGG48150.017.9605467
Produced by FastQC (version 0.11.5)