FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4342445
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2150.032.885831
ACGTCGA1154.1062794E-628.6916921
CGATACG1057.360611E-526.8921972
GCTGGGA69600.026.3326269
CCCGAGT12400.024.7085721
CCCAAAG64150.024.4682851
TACTCGG8600.024.0726243
CGAGTAG9350.023.6513233
CCGAGTA9800.023.5306722
GAGTAGC22450.022.6345374
CTACTCG9000.022.4848632
CGTTCGA1154.114554E-922.47520412-13
GGAGGCT52200.021.786319
GTGCTGG49600.021.6019027
TCGCGGG1100.003041892321.3865875
AGTAGCT37300.020.6870825
TGCTGGG57500.020.6771898
GTAGCTG38300.020.4911586
GCTACTC19150.020.1837121
CCCGAAT6150.019.9275631
Produced by FastQC (version 0.11.5)