FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4749206
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG10300.036.9618153
GCTGGGA92900.035.767699
CGTCGAG1451.8189894E-1135.675711
CTACTCG10650.035.3057862
CGGAGGT17100.034.6318247
GGCGGAG22450.034.543675
AGTAGCT47300.033.883965
CGAGTAG12100.033.7939833
CCGAGTA12300.033.6266062
AGGCGGA24150.032.3069884
GAGTAGC29850.032.278624
CGCCGTA1755.456968E-1232.2267694
GTAGCTG49500.031.9982076
GCTACTC28950.031.513821
TAGCTGG53850.030.4607527
GTGCTGG63250.029.7978387
CCCAAAG82500.029.4702991
TTGGGAG69600.029.4427386
GCGGAGG20150.028.690036
GGAGGCT75950.028.0322289
Produced by FastQC (version 0.11.5)