FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4982450
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA11300.032.0615842
CGAGTAG11500.031.088293
GAGTAGC26000.028.2248944
CCCGAGT14750.028.1134431
CGTCGAG2700.027.9241261
GCTGGGA84900.027.7866849
AGTAGCT45950.026.61765
GTAGCTG49300.025.451016
TAGCTGG48400.025.3417057
CCCAAAG73950.025.1699471
CTACTCG8250.023.9534472
CGCGACT1000.001733165823.560981
GGGAGGC67150.023.0245178
TACTCGG8250.022.8079953
GGAGGCT63300.022.2719489
GCTACTC23450.022.1041181
CGGAGGT12550.021.7182587
TTGGGAG62950.021.5746026
TTGCGCG550.003063510221.36082514-15
CCCGAAT7800.021.1444721
Produced by FastQC (version 0.11.5)