FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4982450
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG10150.034.7288363
CTACTCG10400.032.538572
CGAGTAG9400.030.9997943
GCTGGGA87050.029.8030649
CCGAGTA10450.028.3350262
GGAGGCT70100.027.8240749
TTGGGAG66550.026.9780336
GGGAGGC77100.026.5170698
CCCAAAG75250.025.9950751
AGTAGCT40450.025.2136765
CGCCGTA1501.1640022E-625.0662594
GTGCTGG65700.024.6802047
CCCGAGT13750.023.9386441
CCAAAGT75450.023.6713872
GAGTAGC24650.023.6428454
CGCGTAC902.1640881E-723.50031538-39
AAAGTGC68400.023.0188544
CTCGGGA15150.022.9569445
TGCTGGG74750.022.8865748
GTAGCTG44000.022.5384886
Produced by FastQC (version 0.11.5)