FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4173865
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA4801.1154666E-497.90879340-345
CGTAACG1405.105325E-475.81732300-309
CGAGTAG26100.063.733433
AGTAGCT94750.062.4751055
GAGTAGC63700.060.7959484
GTAGCTG98900.060.0558626
CCGAGTA28500.059.2918852
GCTGGGA200150.058.2473459
TAGCTGG106650.058.0039337
CCCGAGT32150.052.6115761
AAGTGCT113900.052.4275555
CTACTCG22200.051.2332422
CGCAACT4250.00783795650.924503330-339
TACTCGG23450.048.7683033
GCTACTC52500.048.203571
GGAGGCT130450.048.0682649
AGTGCTG123400.047.9218756
AAAGTGC125250.047.157854
TTGGGAG121700.047.151156
CCCAAAG150850.046.749061
Produced by FastQC (version 0.11.5)