FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4364222
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG11450.041.048722
TACTCGG11250.040.9429053
CGAGTAG11850.038.0765843
CCGAGTA12500.037.976632
GCTACTC29050.035.452911
CCCGAGT14350.034.0828131
GCTGGGA103800.033.3254179
CCCAAAG76800.033.3113061
TTGGGAG75500.032.8064166
AGTAGCT46000.032.1845865
AAAGTGC64900.032.0818334
GAGTAGC30350.032.056184
AAGTGCT67100.031.0296155
GGAGGCT80500.030.7687539
GTGCTGG69500.030.363847
TAGCTGG51150.030.0467347
ACTCGGG15950.029.7619114
CCAAAGT74700.029.6350332
AGTGCTG73500.029.6066196
GTAGCTG49450.029.463926
Produced by FastQC (version 0.11.5)