FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3625270
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGC2202.1370889E-4126.188225330-339
ATCCCGT7400.002631785367.06407320-329
GCGTAGA2653.5138274E-462.42442320-329
CGAGTAG20800.058.0410273
GAGTAGC48900.056.7334
AGTAGCT73700.056.1740235
CCGAGTA23050.054.7644352
GTAGCTG75450.054.256756
GCTGGGA147450.053.6257829
CCGGTTT9451.2914825E-1052.515785320-329
TAGCTGG80750.051.902667
CTACTCG16100.050.7549132
ACGGCGA4000.003322250750.535927340-345
TACTCGG16350.050.3441733
AAGTGCT80000.049.8449335
CGGGGAT8555.442962E-747.28508340-345
CCCGAGT28200.046.550951
GCTACTC41050.046.4066931
AAAGTGC86800.045.4483154
AGTGCTG88550.044.7853666
Produced by FastQC (version 0.11.5)