FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4235182
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8550.038.479763
CTACTCG8900.036.966512
CCGAGTA10900.033.633232
CGGAGGT12200.033.516597
CGAGTAG10450.032.8327263
GCTGGGA76900.031.2305499
CCCGAGT12800.030.4954681
GCTACTC23850.030.3668291
CCCAAAG68750.029.6882211
GGCGGAG17000.029.0295815
GCGGAGG14300.028.9232466
AGGCGGA17450.028.280974
AGTAGCT37400.028.1498975
GAGTAGC25050.027.9562534
CTCGGGA12450.027.5583955
TTGGGAG61300.027.1420866
GCGGTTA3300.027.0598078
GGAGGCT63150.026.5692889
GGGAGGC71700.026.4817858
GTGCTGG56900.026.267297
Produced by FastQC (version 0.11.5)