FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3402189
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG19700.059.3317572
CCGAGTA18750.059.189522
GTCGGTA1755.0462503E-459.042076245
CGAGTAG18100.059.0320973
GCGACGC3200.004619720458.203403230-234
TACTCGG20250.057.7202763
AGTAGCT65750.057.011995
AAGTGCT87400.056.9382865
GCTGGGA149950.056.810329
GAGTAGC42000.056.502154
GCTACTC44850.054.3843271
GTAGCTG70200.053.6502576
CCCAAAG111300.052.797591
TTGGGAG109150.052.515046
AGTGCTG93150.051.775876
AAAGTGC94600.051.731094
CTTCGGT8003.5531409E-451.66182245
CCAAAGT103650.050.85932
GGAGGCT115850.050.499269
TAGCTGG77750.049.809357
Produced by FastQC (version 0.11.5)