FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4195080
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG8950.033.6456683
CTACTCG9700.032.9923362
TACTCGG10050.032.7720953
CCGAGTA9600.031.8652972
GCTGGGA83000.030.6873879
CCCAAAG69350.030.3780171
CGGAGGT13450.029.6985577
GCTACTC22200.027.598711
CCCGAGT13100.027.3426531
GAGTAGC25600.027.0174524
GTGCTGG57300.026.900357
AAAGTGC56750.026.7795754
AGTAGCT41450.025.994295
GGAGGCT59500.025.5897949
TAGCTGG43950.025.5551077
GGGAGGC72650.025.3564918
AAGTGCT59950.024.8792385
TTGGGAG65200.024.7956476
CCAAAGT69350.024.7697852
CGGCCTA4000.024.671648
Produced by FastQC (version 0.11.5)