FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5868504
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA15350.073.690581
CGTCGAG35350.055.0642551
CGCGACT3650.051.6507071
GTCGAGA62100.034.532571
CGAGACT116300.030.3131471
GACTTGT148750.028.717124
TCGAGAC82250.028.6089362
GTGATCA153950.028.0218899
ACTTGTG159250.027.0895565
TGTGATC160000.027.0210518
AGACTTG153600.026.6771183
GCGACTT7400.026.0747262
CTTGTGA167050.025.8532146
CGACTTG7850.024.571193
GAGACTT146650.024.0683772
TTGTGAT187850.023.3151977
CCGTCGA1903.5484118E-722.3253381
ACGTCCA12900.020.4600851
CGTCCAG14150.019.9554922
GCTGGGA118950.018.7656489
Produced by FastQC (version 0.11.5)