FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3535834
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11400.043.7474753
TACTCGG9350.041.765573
CTACTCG9650.041.44992
CCGAGTA12400.040.2268562
CCCGAGT14000.039.3882521
GCTACTC24450.039.3242261
CGTACCG1209.640644E-1139.207643
GCTGGGA92000.038.5146069
AGTAGCT45300.037.3900685
CCCAAAG68100.037.1651651
AAAGTGC55800.036.931074
GTAGCTG45400.036.7479256
AAGTGCT57450.036.116075
CCAAAGT62850.035.9391562
GAGTAGC28450.035.886364
CGGAGGT14750.034.4091267
AGTGCTG58650.034.055036
TAGCTGG50150.033.9218527
GTGCTGG58300.033.6131677
CAAAGTG64400.033.0951463
Produced by FastQC (version 0.11.5)