FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3267761
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC1600.0034212999184.95143320-329
CGCCGAA2457.0936094E-6113.82669240-249
ACGGTCG2003.6173602E-589.4116340-345
AGTAGCT72000.064.403745
CGAGTAG22250.061.2145163
GTAGCTG77900.059.3115586
CCGAGTA23700.058.787152
GAGTAGC50050.057.518914
GCTGGGA160600.056.267069
TAGCTGG85450.055.8797957
AAGTGCT82400.054.9978375
CCCGAGT27850.052.090451
AGTGCTG90700.050.19116
TTGGGAG101850.049.8583956
CCAAAGT97750.049.4746252
AAAGTGC91750.049.325684
TACTCGG16950.048.5784573
CCCAAAG112800.047.4867131
CAAAGTG97850.047.389573
CTACTCG17300.047.2471962
Produced by FastQC (version 0.11.5)