FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3267761
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG3851.1897982E-5102.5589245
CCGATTA2955.3084607E-4100.38604245
AGTAGCT72250.067.331945
CGAGTAG18950.065.6647953
GTAGCTG75950.063.8177346
CCGAGTA19900.063.721092
CTACTCG21750.062.66012
GAGTAGC45700.061.8459664
TACTCGG22200.060.3223083
TAGCTGG83950.059.07917
GCTGGGA168600.058.761739
AAGTGCT90100.058.5303425
AAAGTGC95250.056.422394
CCAAAGT103400.056.3893432
GCTACTC48950.056.3121721
TTGGGAG117150.055.5361636
CCCAAAG116200.054.483761
AACGCGA1152.9561887E-453.645775235-239
CAAAGTG103650.053.5664443
CCCGAGT25200.052.4573441
Produced by FastQC (version 0.11.5)