FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632450
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG7800.041.6152733
CGAGTAG9600.040.6728973
CTACTCG7700.039.1076552
CCGAGTA10500.037.1930122
GAGTAGC21600.036.153694
GCTGGGA70250.035.3884779
AGTAGCT33500.034.966555
GCTACTC18200.034.700311
CCCAAAG50950.034.04111
TAGCTGG36650.033.467187
GTAGCTG35750.033.2593356
TCGGGAG10300.032.8521926
CCCGAGT12750.032.529131
CCAAAGT47750.032.221592
AAAGTGC42600.032.024824
AAGTGCT44800.030.767195
GCGCTAC1401.8426363E-830.2980331
GGAGGCT54150.029.6811169
CAAAGTG48450.029.6144943
CTCGGGA11000.029.5090085
Produced by FastQC (version 0.11.5)