FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632450
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9350.047.7547723
CTACTCG9650.045.783112
CGAGTAG8350.045.593543
CCGAGTA9250.042.681762
GCTACTC21600.041.1459731
AGTAGCT33400.038.8389435
GCTGGGA73650.038.3521779
CCAAAGT49300.037.5624662
AAAGTGC44450.037.4314164
GGAGGCT58200.037.389219
GAGTAGC21750.037.3845024
CCCAAAG52700.037.0302661
CCCGAGT11800.036.2642481
AAGTGCT47000.035.800575
TTGGGAG54400.035.423366
GTAGCTG35250.034.5338866
AGTGCTG48500.034.402616
ACTCGGG13000.034.3467034
TAGCTGG36200.034.276797
CAAAGTG50700.034.2076453
Produced by FastQC (version 0.11.5)