FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3406263
Sequences flagged as poor quality0
Sequence length35-351
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA4250.002226531369.967064270-279
AGTAGCT66900.057.9247785
CGAGTAG19450.055.5717663
GTAGCTG70250.055.126096
CCGAGTA20500.054.5340772
GCTGGGA146500.052.741149
GAGTAGC45800.052.42914
TAGCTGG75200.051.9855887
AAGTGCT79500.047.3537645
CGGTCAC7655.671907E-446.992306260-269
CCCGAGT25900.045.1070821
ATAGCGG4309.7398495E-445.034657340-345
AGTGCTG84900.044.890876
ACGCACG3700.00412881943.950527320-329
TAAGGCG3701.6462684E-543.950527320-329
CTACTCG14450.043.359352
AAAGTGC85750.043.2577484
TACTCGG14900.042.453363
TTGGGAG88600.041.907896
GTGCTGG94150.040.8703737
Produced by FastQC (version 0.11.5)