FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3406263
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA3152.1847882E-867.478134245
CGCGTTC1950.0028200265.931564185-189
CGAGTAG16500.061.9088063
TTGCGTG5300.00405093360.157394245
AGTAGCT61500.058.9490935
CCGAGTA17900.055.417452
GAGTAGC40100.054.9222534
GTAGCTG67150.053.989126
CTACTCG19800.053.3799442
TACTCGG20200.051.73833
GCTGGGA149750.051.0599569
TAGCTGG74050.050.3951387
AAGTGCT80350.050.116695
GCTACTC44950.049.672751
TTGGGAG101000.048.2299086
AAAGTGC83250.047.732564
CCAAAGT93000.047.5542262
CCCAAAG102850.046.7494161
CAAAGTG93000.045.6495173
AGTGCTG88850.045.322186
Produced by FastQC (version 0.11.5)