FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4583315
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT952.1194865E-839.5785874
TCGCGTT550.002717973734.1815035
CTACTCG9250.034.042932
TACTCGG9050.033.7565963
GCTGGGA82750.031.1812469
CGAGTAG10300.029.2036153
CCCAAAG71650.028.3563631
CGGAGGT13650.028.2341737
CCGAGTA10600.027.9337062
TTGGGAG65700.027.8992866
GGAGGTT42100.027.0160858
AGGCGGA18750.026.5704234
GGAGGCT65450.026.2821319
GGCGGAG19350.025.9894245
AAAGTGC61100.025.8459174
GGGAGGC78350.024.954198
TGCTGGG67150.024.8468598
GTGCTGG60600.024.7406967
GAGTAGC24450.024.6050914
CCAAAGT71300.023.8623332
Produced by FastQC (version 0.11.5)