FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4107596
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA12250.044.1759572
CGAGTAG11400.044.158383
CCCGAGT13850.040.152571
CGTCGAG2000.037.7025831
GCTGGGA97000.034.8821379
CGGAGGT14700.034.5261967
GAGTAGC29100.033.46664
AGTAGCT46500.033.084845
TACTCGG9250.033.060233
CCCAAAG71800.032.490911
GTAGCTG47700.031.7237916
CTACTCG9650.031.2087842
TAGCTGG51350.031.20737
GCTACTC23650.030.6881481
AAAGTGC61250.030.4174864
CCAAAGT67450.030.2783342
AAGTGCT62000.030.1254165
AGTGCTG64200.029.2800986
TTGGGAG70950.029.0114386
GGAGGCT70850.028.9193769