FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4670728
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG4150.056.7876241
CGCGACT804.5292836E-947.1337281
GTCGAGA8300.029.4858762
GCTGGGA75300.026.5865129
CGGAGGT10200.025.3401537
TACTCGG9200.024.0386663
GGCGGAG11750.024.0277825
CCCAAAG64050.022.9597531
ACGTCGA1852.7424176E-722.9299221
CCCGAAT7400.022.9299221
CGAGTAG11300.022.902593
CCGAGTA11250.022.5907152
GAGTAGC26900.022.5651254
CCCGAGT13850.022.4608361
GTGCTGG50350.022.213877
CTCGGGA10450.022.063795
AGTAGCT42700.022.0395345
CGCGCCC5800.021.90912
TAGCTGG46300.021.5179827
CTACTCG9600.021.0807462
Produced by FastQC (version 0.11.5)