FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4670728
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG301.3715739E-462.66695
GCTGGGA86800.035.1410879
CGGAGGT15450.034.983957
CTACTCG10350.034.5125772
TACTCGG10450.034.181953
GCTACTC26500.032.2983861
GGCGGAG19250.032.228695
CCGAGTA11250.032.1693532
CGAGTAG11600.031.6035673
AGTAGCT45300.030.6071785
CCCAAAG74650.030.4909421
GCGGAGG17300.030.4278586
GTGCTGG58900.030.402497
TTGGGAG72400.029.9918256
GAGTAGC29950.029.97344
CCCGAGT14200.029.8063931
AGGCGGA20050.029.7706874
CGTAGCG953.754341E-529.6846392
GGGAGGC80550.029.5239968
GGAGGCT72500.029.4960339
Produced by FastQC (version 0.11.5)