FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2835001
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG9350.047.2956663
CTACTCG8600.045.958162
TACTCGG9300.042.491443
CCGAGTA10750.042.4565852
AGTAGCT38550.039.050875
CCCAAAG59400.038.72081
GCTACTC20600.038.6661031
GCTGGGA85600.038.6502089
AAAGTGC48100.038.4372754
CCCGAGT12400.038.389341
GAGTAGC24350.038.0603144
GTAGCTG40250.037.596786
AAGTGCT49150.037.5204165
CCAAAGT55050.037.009432
TCGGGAG11750.036.7968446
TAGCTGG42750.036.6067437
AGTGCTG51500.035.4066736
CAAAGTG55300.035.3043373
ACTCGGG12100.033.8250924
TTGGGAG53100.033.5432626
Produced by FastQC (version 0.11.5)