FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3171847
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9450.047.746432
CGAGTAG10950.045.4980963
GCTGGGA89250.044.813539
CCGAGTA11550.044.762282
GCTACTC26150.044.4229661
GAGTAGC25100.044.378164
CCCAAAG67750.043.5945931
TACTCGG10050.042.557573
AGTAGCT42100.042.3108525
AAAGTGC56800.041.455684
CCAAAGT64750.041.15712
AAGTGCT57200.041.0836145
GTAGCTG43400.039.8522456
CCCGAGT14000.038.9683721
GTGCTGG59150.038.9346247
TTGGGAG65700.038.9166
TAGCTGG45750.038.83257
GGAGGCT72800.038.218899
CAAAGTG65900.038.0139773
TCGCGAA757.449724E-637.59853794
Produced by FastQC (version 0.11.5)