FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4407556
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA88300.031.29289
CCCAAAG67600.030.7574841
CGAGTAG11450.030.414123
CCCGAGT13900.030.1879841
CCGAGTA12850.028.572232
GAGTAGC27700.027.8617364
CGATACG856.759338E-427.6887762
AAAGTGC57500.027.4170724
AGTAGCT44400.027.3451845
GTGCTGG59950.026.7296357
TAGCTGG50200.026.5853547
TTGGGAG69400.026.2733156
GTAGCTG45800.026.1647936
ACGATCG909.5309364E-426.1069038
GGAGGCT64300.025.7983289
CGGGTCG1101.0200957E-425.6322299
CCAAAGT69300.025.53922
AAGTGCT62350.025.284395
TACTCGG9900.025.1935713
GGGAGGC79100.025.0111398
Produced by FastQC (version 0.11.5)