FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4407556
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG12400.040.1782653
CTACTCG11800.039.4330332
GCTACTC27650.037.0787121
GCTGGGA97900.036.2937059
CGAGTAG9950.035.427883
TTGGGAG77050.033.7325866
AAAGTGC62700.033.0577354
AGTAGCT46650.032.8445325
CCCAAAG79800.032.6490171
CCGAGTA11350.032.3002362
GGAGGCT78700.032.1291859
GTGCTGG63500.032.0487637
CGGAGGT17200.031.9708757
CCCGAGT13050.031.7128641
GAGTAGC25900.031.2126584
TAGCTGG52950.030.8007037
GTAGCTG50200.030.7091126
AAGTGCT68200.030.5981885
TCGGGAG17250.030.515896
GGGAGGC97200.030.4625668
Produced by FastQC (version 0.11.5)