FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4041592
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1750.040.4042051
CGAGTAG12650.040.167743
TACTCGG11100.038.1472933
GCTGGGA103050.037.7125249
GAGTAGC31400.037.309024
CCGAGTA13900.036.908182
AGTAGCT52850.036.58825
GTAGCTG51500.036.499446
CTACTCG11100.036.041842
CCCAAAG74250.035.3616141
AAAGTGC60800.034.8993344
CCCGAGT15900.034.686631
CGTAACG550.002704154234.2169654
TAGCTGG58300.033.6120957
CCAAAGT69700.033.4259222
AAGTGCT64400.033.0214965
AGTGCTG65950.032.777556
CAAAGTG69900.032.3751683
GTGCTGG64500.032.2754337
TTGGGAG78650.032.1452646
Produced by FastQC (version 0.11.5)