FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4041592
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG14150.061.119453
CTACTCG15400.056.768872
GCTACTC34150.052.7420731
GCTGGGA128900.049.040539
AAAGTGC78700.048.672634
CCGAGTA14200.047.995192
CGAGTAG13950.047.8445173
CCCAAAG95950.047.639861
AAGTGCT82750.047.3122445
TCGGGAG19950.046.1752976
TTGGGAG95350.045.9400836
GTGCTGG83400.045.70377
CTCGGGA19450.045.670815
AGTAGCT54400.045.099135
AGTGCTG86950.044.9187856
CCAAAGT92750.044.595072
CAAAGTG92000.043.477023
TCGCGTT652.7867318E-643.3844835
ACTCGGG19600.042.9238744
CCCGAGT16250.042.830931
Produced by FastQC (version 0.11.5)