FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3844519
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10350.035.910413
GCTGGGA82950.033.991579
CCCAAAG66800.032.8050161
CCGAGTA11750.032.442672
CCCGAGT12600.031.7915781
AAGTGCT56300.029.4145555
AAAGTGC55400.029.382884
AGTAGCT42950.029.2466475
CGTCGAG1005.2301693E-528.2758061
CCAAAGT65250.028.2010732
CTACTCG9700.028.1400992
GTAGCTG42800.027.9983716
TAGCTGG46550.027.8628627
GAGTAGC27200.027.847424
CAAAGTG65600.026.8943523
CGGAGGT12100.026.7978557
GTGCTGG60950.026.6771077
GGAGGCT62550.026.6708819
GCTACTC23900.026.4222981
TTGGGAG57550.026.2934076
Produced by FastQC (version 0.11.5)