FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3844519
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9950.043.9296573
CTACTCG10150.042.6009942
CGAGTAG11250.041.7777823
CCGAGTA13000.038.684622
GAGTAGC28500.037.1047824
CCCGAGT15300.036.5736351
CCCAAAG73700.035.9852261
GCTGGGA93750.035.794749
GCTACTC26250.034.752421
TCGGGAG13950.034.365156
AAGTGCT62250.033.8244635
AAAGTGC62450.033.716144
AGTAGCT44250.032.6074875
GTGCTGG61700.032.2978027
CCAAAGT71850.032.1837232
TTGGGAG69400.031.7618396
AGTGCTG64550.031.6727146
CTCGCGT1052.0672378E-631.3329324
TCGCGTT600.00416244631.3329325
CAAAGTG69450.030.927293
Produced by FastQC (version 0.11.5)