FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4930661
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG10400.027.156362
CGAGTAG10700.026.8275283
GCTGGGA79550.026.8192429
CGTCGAG2305.456968E-1226.6448781
TACTCGG10300.026.0418723
CCCGAGT15000.024.827561
CCGAGTA11200.024.3760662
CCCAAAG73650.023.7464871
CCGAACG1601.9886156E-623.5704671
GCTACTC24300.023.473471
ACCGCGC4450.023.3056321
CTCGGGA13400.023.1778815
TTGGGAG62950.022.843646
CCCGAAT7900.022.6753881
GGGAGGC70350.022.644698
CGGAGGT13100.021.8821187
AGTAGCT43500.021.7440975
GAGTAGC27050.021.3979634
TAGCTGG47800.021.1369047
TCGCTCG1354.038301E-420.9204562
Produced by FastQC (version 0.11.5)