FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4930661
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG10600.036.358593
CTACTCG11000.033.754642
CGGAGGT15800.033.6136747
AGGCGGA18950.032.2425044
GCTGGGA86650.031.4590239
CTCGCGT1501.0713848E-931.33314
TCGCGTT953.7549427E-529.683995
GCTACTC28700.029.6598421
CCGAGTA12300.029.4228442
GGCGGAG21050.029.2491875
GAGTAGC27000.028.5479374
GGAGGCT73550.027.9883489
CGAGTAG11900.027.6471333
TTGGGAG67850.027.6387026
GCGGAGG18950.027.530146
CCCAAAG79700.027.1438661
GTGCTGG59900.026.442217
AGTAGCT43900.026.3369335
GGGAGGC82750.026.182968
TCGGGAG15650.026.1276046
Produced by FastQC (version 0.11.5)