FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4798399
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8500.034.3195573
GCTGGGA92900.031.3624929
CTACTCG8650.030.4690322
CCCAAAG74600.029.0569441
GAGTAGC29700.028.3573424
CGAGTAG11300.028.3138793
CGTCGAG2500.028.2736721
CCGAGTA11950.027.5687392
TTGGGAG69550.027.2308126
AGTAGCT49400.027.1448125
GGAGGCT66600.027.0951529
ACGATCG1406.383525E-726.8532318
GTGCTGG64000.025.919667
CCAAAGT74900.025.8882182
GTAGCTG49300.025.4517146
GCTACTC24550.025.3368971
AAGTGCT66350.025.0324135
CCCGAGT15300.024.947361
TAGCTGG52050.024.7379917
AAAGTGC67850.024.6870424
Produced by FastQC (version 0.11.5)