FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4798399
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG11200.039.446823
CCGAGTA12700.035.527912
CTACTCG11950.035.397842
GCTGGGA97600.034.7195439
CGAGTAG12700.034.047583
GCTACTC29550.033.74021
CCCGAGT14900.032.825881
CCCAAAG82200.031.4101331
GAGTAGC28600.031.3880944
CGGAGGT19700.031.0152117
GGAGGCT80200.030.3559579
TTGGGAG76450.030.0012916
AGTAGCT47650.029.590755
TAGCTGG51050.028.3564977
GGGAGGC95900.028.2778118
AAAGTGC71650.028.2065454
AAGTGCT73800.028.085355
GTGCTGG70200.027.650987
GTAGCTG50350.027.630576
CCAAAGT81500.027.5082472
Produced by FastQC (version 0.11.5)