FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4057790
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG15500.053.0643273
CTACTCG15450.052.3240852
GCTACTC36050.051.520591
CCGAGTA16950.050.466522
CGAGTAG16050.050.0745933
CCCAAAG101750.049.9089431
AGTAGCT62550.049.3667345
AAAGTGC83650.049.3315664
CCAAAGT92600.048.7261282
GCTGGGA146400.048.668639
GAGTAGC40300.046.9998364
AAGTGCT89000.046.8414085
GTAGCTG65600.046.4983146
CCCGAGT19850.045.4809721
AGTGCTG89900.045.379156
CAAAGTG96350.044.9754523
TAGCTGG71500.044.4362077
GTGCTGG92000.044.0878877
TTGGGAG106550.043.7577066
TCGGGAG21700.042.0182846
Produced by FastQC (version 0.11.5)