FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3774942
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA12300.038.644182
CGAGTAG12150.037.950693
CCCGAGT14900.035.426011
GCTGGGA90300.034.347629
GAGTAGC29200.034.3215334
AGTAGCT46900.033.3069765
CCCAAAG69700.031.6448861
GCTACTC23150.031.3516351
TAGCTGG49900.031.078057
GTAGCTG49050.031.0425856
CTACTCG8750.030.1195052
AAAGTGC56750.029.8474034
TACTCGG8850.029.7724573
CCAAAGT66050.029.3556882
GTGCTGG60750.028.931157
TTGGGAG61450.028.8317746
AAGTGCT61600.028.7195135
CAAAGTG65250.028.2667243
AGTGCTG63300.027.9891386
GGAGGCT65150.027.9149559
Produced by FastQC (version 0.11.5)