FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5112401
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1900.044.6555861
CGCGACT600.00410367331.4243011
CGGAGGT12250.030.305447
GCTGGGA86300.027.2807669
TACTCGG9700.027.1603073
CCGAGTA11450.027.1240562
CCCGAAT6550.026.6266961
CTACTCG9550.026.6076552
CCCGAGT14350.026.278161
ATGCGCG909.468522E-426.1364064
GGCGGAG14950.025.4895525
AGGCGGA15450.024.9691474
CGAGTAG10950.024.9190943
ACGCGAT950.001297856424.7608053
CTCGGGA12100.024.1059725
CCCAAAG73750.023.5202881
AGTAGCT44850.023.3916475
GCTACTC25400.023.0114941
GAGTAGC27000.022.651554
GGAGGCT62500.022.330899
Produced by FastQC (version 0.11.5)