FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3784474
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12450.043.466773
CCGAGTA13150.041.1643872
CCCGAGT14550.039.5251271
GCTGGGA95000.038.880919
GAGTAGC30800.036.51544
CCCAAAG70050.035.867041
AGTAGCT48850.035.4496655
AAAGTGC58850.035.263144
AAGTGCT61500.034.5088355
GTAGCTG48850.034.4398846
TACTCGG9700.033.4738963
GTGCTGG63400.032.984377
AGTGCTG63900.032.87386
TAGCTGG53650.032.8472487
CCAAAGT70150.032.5434532
CAAAGTG66450.032.4339143
GCTACTC24300.031.2317351
GGAGGCT63800.031.157119
CTACTCG10750.030.650612
TGCTGGG71350.030.4288468
Produced by FastQC (version 0.11.5)