FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3784474
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11900.039.495393
TACTCGG11450.038.9952353
CCGAGTA13050.038.5360032
CCCAAAG71150.037.543661
CTACTCG11950.036.970332
CCCGAGT14600.036.7210541
GCTACTC27550.036.5304761
GCTGGGA93600.035.7015579
AAAGTGC59450.035.4176334
GAGTAGC27600.035.0793534
CCAAAGT69800.034.205952
AAGTGCT61900.034.0917365
TTGGGAG70600.034.0180636
GGAGGCT74750.033.324079
AGTAGCT44550.032.9154855
CAAAGTG69400.032.3037033
GTGCTGG63350.032.0501827
AGTGCTG64450.031.9407126
GTAGCTG46150.031.1632776
TAGCTGG49400.030.8255927
Produced by FastQC (version 0.11.5)