FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3678093
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG12900.054.2889253
CTACTCG13150.053.9716722
CGAGTAG12400.045.106593
GCTACTC29850.045.056851
CCCAAAG79500.044.0684971
GCTGGGA115650.043.2807779
AAAGTGC64200.043.2662664
CCGAGTA13000.043.0247462
GAGTAGC32100.041.582474
AGTAGCT51250.041.5427675
TCGGGAG19000.041.309886
AAGTGCT67950.041.154625
TTGGGAG86850.040.7489286
CCAAAGT74700.040.520982
GTAGCTG52500.040.2850846
CCCGAGT14850.039.9009481
CAAAGTG75950.038.92583
ACTCGGG18000.038.9054764
GGAGGCT88100.038.783739
AGTGCTG72350.038.716746
Produced by FastQC (version 0.11.5)