FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3927519
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGA3509.797905E-486.04502245
CGAGTAG24750.073.118633
AGTAGCT91000.069.702885
CCGAGTA26400.068.998212
GTAGCTG94400.067.380976
GAGTAGC61500.066.08914
GCTGGGA213500.064.7279059
TGGTCGA4750.003290931263.401592245
AAGTGCT117300.062.8763735
CCCGAGT30050.061.8272931
CTACTCG28050.060.70892
TAGCTGG109350.060.2858247
TTGGGAG155200.059.7560236
GTGTCGA5100.004361307759.050507245
AAAGTGC122250.058.874384
TACTCGG29600.058.73263
AGTGCTG127800.057.6640556
CCCAAAG145600.057.442051
CCAAAGT134450.056.3551332
GCTACTC61650.056.228971
Produced by FastQC (version 0.11.5)