FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4108155
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7500.031.9957922
TACTCGG7400.031.1507823
CCGAGTA10300.029.236592
CGAGTAG10450.028.811663
CCCAAAG67350.028.7595461
CCCGAGT12700.028.5735931
GCTGGGA78200.028.244619
GCTACTC20600.027.4531291
GAGTAGC25550.027.0664334
CGGAGGT11950.026.7414867
AGTAGCT39300.026.69425
AAAGTGC55900.026.3412954
GTGCTGG56350.025.519497
GGAGGCT55500.025.4869589
CGTCGAG1859.766154E-925.4745241
GTAGCTG41150.024.7821886
TAGCTGG42750.024.294097
AAGTGCT59200.024.2372155
CCAAAGT68000.024.218272
CAAAGTG66500.023.981853
Produced by FastQC (version 0.11.5)