FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4108155
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9800.041.2446943
CTACTCG10050.039.751052
CGAGTAG8700.038.8963623
GCTACTC24650.036.245971
CCGAGTA10100.033.5047842
GCTGGGA84300.032.7266049
GAGTAGC22800.031.539324
GGAGGCT68200.030.7355469
CCCGAGT12150.030.1884921
GGGAGGC74600.029.8627628
TTGGGAG63400.029.8011156
CGGAGGT13100.029.7784797
TAGCTGG43100.029.4430127
GTGCTGG59050.029.3698797
AGTAGCT38300.029.3288365
CCCAAAG72150.029.0032561
ACTCGGG13750.028.7125854
TCGGGAG15750.028.6474786
AAAGTGC60100.028.5439534
CTCGGGA14550.028.4259785
Produced by FastQC (version 0.11.5)