FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993568
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG9750.048.730633
CTACTCG7750.047.9668662
GAGTAGC25900.045.7705354
CCGAGTA11100.044.9364552
AGTAGCT42450.044.105285
TACTCGG8450.043.9800383
CCCGAGT11850.042.9483451
CCCAAAG60450.042.173731
GCTGGGA88550.042.138769
GTAGCTG44450.041.2341586
TAGCTGG45850.041.2037287
CCAAAGT55200.040.492122
AAAGTGC52450.039.642594
AAGTGCT55650.038.0393035
CAAAGTG57500.037.7153663
GTGCTGG58450.036.5827757
AGTGCTG58750.035.837236
GCTACTC19800.035.6999051
GTCGGTA801.1601493E-535.2460987
TTGGGAG59550.033.9352466
Produced by FastQC (version 0.11.5)