FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2723031
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG9900.056.494493
GCTACTC27450.055.50351
CTACTCG11750.054.799562
TACTCGG11750.054.3995633
AAAGTGC56850.053.3241124
CCCAAAG70500.052.4932371
GAGTAGC25250.051.9322554
CCAAAGT65400.051.527112
AGTAGCT43050.050.6569675
GCTGGGA100300.050.607779
CAAAGTG63850.050.4224663
AAGTGCT60850.049.7415925
GTAGCTG44350.048.536256
CCGAGTA11750.048.3996122
TAGCTGG47200.047.4974987
CCCGAGT12450.046.834951
AGTGCTG64600.046.4175836
TTGGGAG71200.045.547396
TCGGGAG15700.045.203466
GTGCTGG67200.043.9222687
Produced by FastQC (version 0.11.5)