FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4156212
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9900.022.8191572
CGTCGAG1452.4811756E-522.7531471
TACTCGG10200.022.141873
GCTGGGA95700.020.624469
CCGAGTA12200.020.4460582
CCCAAAG72650.019.6570551
AAAGTGC62500.019.3475674
GCTACTC25100.019.3408221
CGAGTAG11700.018.9010183
AAGTGCT65700.018.8349135
AGTGCTG67200.018.7424536
CAAAGTG68500.018.5458353
GTGCTGG67600.018.2832857
CCAAAGT71550.018.1548652
TGCTGGG74200.017.607038
TCGGGAG16050.017.5685986
TTGGGAG59000.017.4442776
CCCGAGT17500.017.236671
ACTCGGG15500.016.9992434
GGAGGCT62750.016.7756589
Produced by FastQC (version 0.11.5)