FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4156212
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12600.029.4687823
CTACTCG10350.029.0633242
TACTCGG10800.027.417163
CCGAGTA13050.027.3721372
GAGTAGC27500.026.149564
CCCAAAG70200.023.3831141
CCCGAGT17800.023.2528761
GCTGGGA96750.022.6370439
GCTACTC26550.021.9670221
AAAGTGC60250.021.6086864
AGTAGCT47050.021.5771735
CCAAAGT69450.021.3178752
AAGTGCT62050.021.2845735
GTAGCTG48350.021.094236
GGAGGCT70700.019.6769169
TAGCTGG53350.019.6458517
CAAAGTG70150.019.6311443
AGTGCTG66100.019.6249266
GTGCTGG65500.019.2306487
CTCGGGA15200.018.2435285
Produced by FastQC (version 0.11.5)