FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3884392
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG12100.052.891512
TACTCGG12300.052.4066353
CGAGTAG15650.049.9073643
CCGAGTA16800.047.898212
GAGTAGC38800.044.0195664
GCTACTC31450.043.461471
GCTGGGA126350.042.73569
AGTAGCT61650.042.128585
AAAGTGC69250.041.85354
CCCGAGT20850.041.142671
CCCAAAG84700.040.9563751
CCAAAGT77000.040.457562
GTAGCTG64400.040.2083636
TAGCTGG67450.039.9224977
CAAAGTG77850.038.8013883
AAGTGCT76300.038.6029625
ACTCGGG17200.037.7503934
TCGGGAG19450.037.6925626
AGTGCTG78650.037.2852026
CTCGGGA18900.036.346445
Produced by FastQC (version 0.11.5)