FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3855798
Sequences flagged as poor quality0
Sequence length35-251
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA3457.156032E-493.122856245
CTAAGCG3900.001164655382.377914245
AGTAGCT97950.073.841045
CGAGTAG26350.072.001143
GAGTAGC61550.069.710124
GTAGCTG103950.068.624266
AAGTGCT113400.066.766395
CCGAGTA29050.065.912832
CTACTCG30700.064.461972
GCTGGGA232150.064.173279
TTGGGAG154850.062.880476
TAGCTGG117450.062.4776157
AAAGTGC122650.062.112584
TACTCGG32200.062.002163
CCAAAGT130150.061.0010032
GCTACTC65250.059.4259721
GAGACGT9057.682704E-659.166454245
CCCAAAG146200.058.9559561
CAAAGTG132050.058.7078633
AGTGCTG128750.058.624926
Produced by FastQC (version 0.11.5)